Difference between revisions of "Part:BBa K4432014:Design"

 
 
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===Design Notes===
 
===Design Notes===
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to do
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This sequence has been specifically designed to be used with [[Part:BBa_K4432040|BBa_K4432040]] using the [https://www.denovodna.com/ Salis Lab RBS Library Calculator v2.0] [1-3].
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===Source===
 
===Source===
  
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to do
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DNA synthesis
  
 
===References===
 
===References===
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[1] Reis AC, Salis HM. An automated model test system for systematic development and improvement of gene expression models. ACS synthetic biology (2020) 9: 3145–3156.
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[2] Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM. Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria. Molecular Systems Biology (2014) 10: 731.
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[3] Ng CY, Farasat I, Maranas CD, Salis HM. Rational design of a synthetic Entner-Doudoroff pathway for improved and controllable NADPH regeneration. Metabolic Engineering (2015) 29: 86–96.

Latest revision as of 21:30, 26 September 2022


Synthetic RBS designed for Pseudomonas aeruginosa PhzA1-G1 operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence has been specifically designed to be used with BBa_K4432040 using the Salis Lab RBS Library Calculator v2.0 [1-3].

Source

DNA synthesis

References

[1] Reis AC, Salis HM. An automated model test system for systematic development and improvement of gene expression models. ACS synthetic biology (2020) 9: 3145–3156.

[2] Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM. Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria. Molecular Systems Biology (2014) 10: 731.

[3] Ng CY, Farasat I, Maranas CD, Salis HM. Rational design of a synthetic Entner-Doudoroff pathway for improved and controllable NADPH regeneration. Metabolic Engineering (2015) 29: 86–96.