Difference between revisions of "Part:BBa K4329002"

Revision as of 21:17, 26 September 2022

sgRNA for targeting Bifunctional BirA enzyme

what is it?

CRISPR/dCas9 is a gene editing technology used to inhibit the expression from the targeted sequence inducing DNA to activate its self-repair process.

What it does?

This system insures the maximum yield of biotin. We have designed a sgRNA sequence (AAGTCACCATACGTTAGTAC) targeting the Bifunctional BirA enzyme after the production of biotin to prevent biotin from further disintegration.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 19
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 ===what is it?===
-CRISPR/Cas9 is a gene editing technology used to arbitrarily removes 2-7 genes from the targeted sequence inducing DNA to activate its self-repair process. The newly formed DNA will contain incorrect DNA sequences that could not be translated thereby inhibiting the function of the specific DNA chain.
+CRISPR/dCas9 is a gene editing technology used to inhibit the expression from the targeted sequence inducing DNA to activate its self-repair process.
 ===What it does?===
-This system insures the maximum yield of biotin. We have designed a sgRNA sequence (AAGTCACCATACGYYAGTAC) targeting the Bifunctional BirA enzyme after the production of biotin to prevent biotin from further disintegration.
+This system insures the maximum yield of biotin. We have designed a sgRNA sequence (AAGTCACCATACGTTAGTAC) targeting the Bifunctional BirA enzyme after the production of biotin to prevent biotin from further disintegration.
 <!-- Add more about the biology of this part here