Difference between revisions of "Part:BBa K4432320:Design"
 
Line 11: Line 11:
 
===Source===
 
===Source===
  
−
DNA synthesis & Assembly
+
DNA synthesis, PCR amplification & Assembly
  
 
===References===
 
===References===

Latest revision as of 21:14, 26 September 2022


CrtW, idi and dsx expression operon under the control of the T5 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1290
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1290
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1464
    Illegal BamHI site found at 1471
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1290
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1290
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2225


Design Notes

T5 promoter (BBa_K4432000) + custom made RBS (BBa_K4432010) + CrtW coding sequence (BBa_K4432020) + custom made RBS (BBa_K4432012) + idi coding sequence (BBa_K3166068) + custom made RBS (BBa_K4432013) + dxs coding sequence (BBa_K3166061)

Source

DNA synthesis, PCR amplification & Assembly

References