Difference between revisions of "Part:BBa K4432142:Design"

 
 
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===Design Notes===
 
===Design Notes===
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to do
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T5 promoter ([[Part:BBa_K4432000|BBa_K4432000]]) + custom made RBS ([[Part:BBa_K4432014|BBa_K4432014]]) + ''Pseudomonas aeruginosa'' PhzA1-G1 truncated operon ([[Part:BBa_I723028|BBa_I723028]])
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+
−
 
+
  
 
===Source===
 
===Source===
  
−
to do
+
PCR amplification & Assembly
  
 
===References===
 
===References===

Latest revision as of 20:42, 26 September 2022


Pseudomonas aeruginosa PhzA1-G1 truncated operon under the control of the T5 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1048
    Illegal EcoRI site found at 3670
    Illegal PstI site found at 1191
    Illegal PstI site found at 4675
    Illegal PstI site found at 5224
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1048
    Illegal EcoRI site found at 3670
    Illegal PstI site found at 1191
    Illegal PstI site found at 4675
    Illegal PstI site found at 5224
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1048
    Illegal EcoRI site found at 3670
    Illegal BamHI site found at 397
    Illegal BamHI site found at 1603
    Illegal XhoI site found at 341
    Illegal XhoI site found at 500
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1048
    Illegal EcoRI site found at 3670
    Illegal PstI site found at 1191
    Illegal PstI site found at 4675
    Illegal PstI site found at 5224
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1048
    Illegal EcoRI site found at 3670
    Illegal PstI site found at 1191
    Illegal PstI site found at 4675
    Illegal PstI site found at 5224
    Illegal NgoMIV site found at 263
    Illegal NgoMIV site found at 1272
    Illegal NgoMIV site found at 1419
    Illegal NgoMIV site found at 1437
    Illegal NgoMIV site found at 1441
    Illegal NgoMIV site found at 2245
    Illegal NgoMIV site found at 2419
    Illegal AgeI site found at 5513
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5656
    Illegal BsaI.rc site found at 2355
    Illegal BsaI.rc site found at 4732
    Illegal BsaI.rc site found at 5138
    Illegal BsaI.rc site found at 5165
    Illegal SapI.rc site found at 4152


Design Notes

T5 promoter (BBa_K4432000) + custom made RBS (BBa_K4432014) + Pseudomonas aeruginosa PhzA1-G1 truncated operon (BBa_I723028)

Source

PCR amplification & Assembly

References