Difference between revisions of "Part:BBa K4329001"

Revision as of 20:09, 26 September 2022

Overexpression of dethiobiotin synthase and biotin synthase in E. coli

what is it?

In order to alter the metabolic pathway of E. coli and tend to a more productive way of Biotin, we enhance the metabolic pathway in the E. coli by increasing the expression of dethiobiotin synthase and biotin synthase. This plasmid is regulated by T7 promoter, Lac operator, and T7 terminator. With IPTG, RNA ligase will converge with T7 promoter which will then lead to the production of mRNA. Thus, protein and enzyme production will begin. The plasmid will only enhance the production of Dethiobiotin synthase and biotin synthase in E.coli achieving the goal of creating large amount of biotin.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 ===what is it?===
 In order to alter the metabolic pathway of E. coli and tend to a more productive way of Biotin, we enhance the metabolic pathway in the E. coli by increasing the expression of dethiobiotin synthase and biotin synthase.
+This plasmid is regulated by T7 promoter, Lac operator, and T7 terminator. With IPTG, RNA ligase will converge with T7 promoter which will then lead to the production of mRNA. Thus, protein and enzyme production will begin. The plasmid will only enhance the production of Dethiobiotin synthase and biotin synthase in E.coli achieving the goal of creating large amount of biotin.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
-This plasmid is regulated by T7 promoter, Lac operator, and T7 terminator. With IPTG, RNA ligase will converge with T7 promoter which will then lead to the production of mRNA. Thus, protein and enzyme production will begin. The plasmid will only enhance the production of Dethiobiotin synthase and biotin synthase in E.coli achieving the goal of creating large amount of biotin.