Difference between revisions of "Part:BBa K4432030:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
to do
+
This sequence has been specifically optimised for ''Escherichia coli'' with the help of IDT’s online Codon Optimization Tool based on the original CrtO of ''Shewanella oneidensis'' MR-1 sequence (UniProt Q8E8S0).
−
 
+
  
  
 
===Source===
 
===Source===
  
−
to do
+
DNA synthesis
  
 
===References===
 
===References===

Revision as of 18:55, 26 September 2022


MrtCAB expression cassette followed by the PANTR1 Toehold Switch Trigger 06 Transcriptional Unit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 361


Design Notes

This sequence has been specifically optimised for Escherichia coli with the help of IDT’s online Codon Optimization Tool based on the original CrtO of Shewanella oneidensis MR-1 sequence (UniProt Q8E8S0).


Source

DNA synthesis

References