Difference between revisions of "Part:BBa K200009"
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Dam Sensitive Restriction Enzyme.
 
Dam Sensitive Restriction Enzyme.
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Recognition Sequence and Cutting site
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The recognition sequence of this restriction enzyme is GATC, and this enzyme is a 4 base cutting enzyme leaving a 4 base overhang.
  
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NGATCN  ->  N  GATCN<br>
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<br>
−
NCTAGN     NCTAG  N   
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The Cutting Site is as shown:
 +
<br>
 +
NGATCN  ->  N  GATCN <br>
 +
NCTAGN ->  NCTAG  N   
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:18, 12 August 2009

DpnII Composite

Dam Sensitive Restriction Enzyme. The recognition sequence of this restriction enzyme is GATC, and this enzyme is a 4 base cutting enzyme leaving a 4 base overhang.


The Cutting Site is as shown:
NGATCN -> N GATCN
NCTAGN -> NCTAG N

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]