Difference between revisions of "Part:BBa K4263001"

 
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ACC1 is the major member of ACCs in mammalian, mountains of documents record the roles of ACC1 in various diseases, such as cancer, diabetes, obesity. Besides, acetyl-CoA and malonyl-CoA are cofactors in protein acetylation and malonylation, respectively, so that the manipulation of acetyl-CoA and malonyl-CoA by ACC1 can also markedly influence the profile of protein post-translational modifications, resulting in alternated biological processes in mammalian cells.  
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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    <h2 style="font-weight: bold;">P<sub>ACC1</sub></h2>
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    <h2>Introduction</h2>
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    <p>ACC1 is the major member of ACCs in mammalian<sup>[1]</sup>.</p>
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    <h2>Characterization</h2>
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    <p>In order to test the function of P<em><sub>ACC1</sub></em>, we construct "P<em><sub>ACC1</sub>-EGFP-</em>terminator" (Figure 1). If P<em><sub>ACC1</sub></em> is functional, we can test the fluorescence intensity of EGFP in supernatant samples obtained from the culture of recombinant <em>P.pastoris</em> GS115 strain.</p>
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===Functional Parameters===
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    <h4>Figure 1 Gene circuit of P<em><sub>ACC1</sub>-EGFP-</em>terminator</h4>
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    <p>Our results matched the general expected trend (Figure 2). After fermentation experiment in BMMY medium containing 1% methanol. The fluorescence intensity of the samples of recombinant <em>P.pastoris</em> GS115 containing the <em>EGFP</em> gene gradually increased over time . At the same time, we measured the growth curve of the strains.</p>
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    <img src="https://static.igem.wiki/teams/4263/wiki/parts/image/pacc1-min.jpg" alt="">
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    <h4>Figure 2 Fluorescence intensity and OD600 absorbance of samples obtained at different time points from the culture of corresponding recombinant <em>P.pastoris</em> GS115 containing <em>EGFP</em> gene.</h4>
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    <h2>Reference</h2>
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    <p>[1] Wang Y, Yu W, Li S, et al. Acetyl-CoA Carboxylases and Diseases[J]. Front Oncol, 2022,12:836058.</p>
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Revision as of 15:36, 26 September 2022

K4263001

PACC1

Introduction

ACC1 is the major member of ACCs in mammalian[1].

Characterization

In order to test the function of PACC1, we construct "PACC1-EGFP-terminator" (Figure 1). If PACC1 is functional, we can test the fluorescence intensity of EGFP in supernatant samples obtained from the culture of recombinant P.pastoris GS115 strain.

Figure 1 Gene circuit of PACC1-EGFP-terminator

Our results matched the general expected trend (Figure 2). After fermentation experiment in BMMY medium containing 1% methanol. The fluorescence intensity of the samples of recombinant P.pastoris GS115 containing the EGFP gene gradually increased over time . At the same time, we measured the growth curve of the strains.

Figure 2 Fluorescence intensity and OD600 absorbance of samples obtained at different time points from the culture of corresponding recombinant P.pastoris GS115 containing EGFP gene.

Reference

[1] Wang Y, Yu W, Li S, et al. Acetyl-CoA Carboxylases and Diseases[J]. Front Oncol, 2022,12:836058.