Difference between revisions of "Part:BBa K4220000"

 
Line 3: Line 3:
 
<partinfo>BBa_K4220000 short</partinfo>
 
<partinfo>BBa_K4220000 short</partinfo>
  
−
RTAC:
+
−
1)Introduction: Rspo-Targeting Anti-cancer Chimeric protein which can be expressed in mammalian cells and collected in culture medium.
+
==Introduction:==
−
2)Composition: ZNRF3+linker+LGR4+human IgGH3 Fc
+
RTAC, Rspo-Targeting Anti-cancer Chimeric protein which can be expressed in mammalian cells and collected in culture medium.
−
FZD5 sp: The signaling peptide of FZD5
+
==Composition: ==
 +
ZNRF3+linker+LGR4+human IgGH3 Fc
 +
==The design and improvement of RTAC==
 +
: Better interaction with 4 RSPOs--changing the length of linker
 +
: Better secretion capability--changing the type of signaling peptide
 +
: Better stability--adding an IGg Fc tag
  
  

Revision as of 12:48, 26 September 2022


FZD5 sp-V5-RTAC


Introduction:

RTAC, Rspo-Targeting Anti-cancer Chimeric protein which can be expressed in mammalian cells and collected in culture medium.

Composition:

ZNRF3+linker+LGR4+human IgGH3 Fc

The design and improvement of RTAC

Better interaction with 4 RSPOs--changing the length of linker
Better secretion capability--changing the type of signaling peptide
Better stability--adding an IGg Fc tag


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 70
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1254
    Illegal BamHI site found at 2171
    Illegal XhoI site found at 2162
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2274
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 124
    Illegal BsaI site found at 1451
    Illegal BsaI.rc site found at 1139