Difference between revisions of "Part:BBa K4232000"

Revision as of 07:54, 26 September 2022

It is a widely regarded catalyst that oxidizes many substrates and reduces oxygen to water.

This part of the code is laccase. full name multicopper oxidase CueO, is derived from E. coli. It is a multinuclear copper-containing oxidase widely found in plants and animals, microorganisms, can oxidize many reducing substrates while reducing oxygen to water, so it can play the role of oxygen consumption.

The introduction of a signal peptide needs to be considered in the use of laccase. cueO has 516 amino acid residues, but the first 28 amino acids are signal peptides that will localize laccase to the periplasm. If CueO is desired to function intracellularly, the sequence corresponding to the signal peptide can be removed.

Reference：Modulation of Intracellular O2 Concentration in Escherichia coli Strains Using Oxygen Consuming Devices（ACS Synth. Biol. 2018, 7, 1742−1752）

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 225
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4232000 short</partinfo>
-This part of the code is laccase. It is a reductive enzyme in E. coli. This part is useful for catalyzing intracellular oxygen with reducing substances, accelerating oxygen consumption and thus creating an intracellular hypoxic environment.
+This part of the code is laccase. full name multicopper oxidase CueO, is derived from E. coli. It is a multinuclear copper-containing oxidase widely found in plants and animals, microorganisms, can oxidize many reducing substrates while reducing oxygen to water, so it can play the role of oxygen consumption.
+The introduction of a signal peptide needs to be considered in the use of laccase. cueO has 516 amino acid residues, but the first 28 amino acids are signal peptides that will localize laccase to the periplasm. If CueO is desired to function intracellularly, the sequence corresponding to the signal peptide can be removed.
+Reference：Modulation of Intracellular O2 Concentration in Escherichia coli Strains Using Oxygen Consuming Devices（ACS Synth. Biol. 2018, 7, 1742−1752）