Difference between revisions of "Part:BBa K4232001"
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<partinfo>BBa_K4232001 short</partinfo> | <partinfo>BBa_K4232001 short</partinfo> | ||
| − | This part of the code is '''leghemoglobin'''. It comes from the soybean rhizosphere. It can bind to intracellular oxygen and transport it to the end of the aerobic respiratory chain (such as the cell membrane of E. coli), thus creating a localized hypoxic environment in the cell, yet leaving the process of aerobic respiration providing energy unimpaired ( | + | This part of the code is '''leghemoglobin'''. It comes from the soybean rhizosphere. It can bind to intracellular oxygen and transport it to the end of the aerobic respiratory chain (such as the cell membrane of E. coli), thus creating a localized hypoxic environment in the cell, yet leaving the process of aerobic respiration providing energy unimpaired (The specific mechanism of action is shown in the figure below). |
Since the bean hemoglobin gene is of plant origin, when performing gene recombination on prokaryotes, it is necessary to remove introns from their gene sequences, do codon optimization, and take measures to make them active after expression. | Since the bean hemoglobin gene is of plant origin, when performing gene recombination on prokaryotes, it is necessary to remove introns from their gene sequences, do codon optimization, and take measures to make them active after expression. | ||
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Reference：Andrés R. Schwember, et al.Regulation of Symbiotic Nitrogen Fixation in Legume Root Nodules.(2019)Plants. | Reference：Andrés R. Schwember, et al.Regulation of Symbiotic Nitrogen Fixation in Legume Root Nodules.(2019)Plants. | ||
| − | [[File:BIT-China-parts-leghemoglobin-mechanism.jpeg|200px | + | [[File:BIT-China-parts-leghemoglobin-mechanism.jpeg|200px]] |
Revision as of 07:41, 26 September 2022
It is the "switch" that regulates the oxygen concentration in the rhizosphere
This part of the code is leghemoglobin. It comes from the soybean rhizosphere. It can bind to intracellular oxygen and transport it to the end of the aerobic respiratory chain (such as the cell membrane of E. coli), thus creating a localized hypoxic environment in the cell, yet leaving the process of aerobic respiration providing energy unimpaired (The specific mechanism of action is shown in the figure below).
Since the bean hemoglobin gene is of plant origin, when performing gene recombination on prokaryotes, it is necessary to remove introns from their gene sequences, do codon optimization, and take measures to make them active after expression.
Reference：Andrés R. Schwember, et al.Regulation of Symbiotic Nitrogen Fixation in Legume Root Nodules.(2019)Plants.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]