Difference between revisions of "Part:BBa K4515004"

Revision as of 07:06, 26 September 2022

thl-opt

Sequence and Features

Assembly Compatibility:

@@ Line 8: / Line 8: @@
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+BBa_K4515004 is an enzyme named thl, which encodes acetyl-CoA acetyltransferase and converts Acetyl-CoA into Acetoacetyl-CoA in the N-butanol biosynthesis pathway [1-2]. It has been found that N-butanol can be synthesized naturally in Clostridium, but the tolerance of Clostridium bacteria is not good enough for large-scale production [3-5]. Recently, Lactobacillus Brevis with better N-butanol tolerance has been isolated by researchers. In order to improve the yield of N-butanol, we optimized the codon of the thl gene and inserted the optimized thl DNA fragment downstream of the Pthl promoter (Figure 1), and transformed it into L. brevis ATCC367 competent cells to complete the N-butanol metabolic pathway [6].
+[[File:T--East China--BBa K4515004-Figure1.png|500px|thumb|center|Figure 1. the plasmid map of the composite part BBa_K4515005..]]
 <!-- -->