Revision as of 06:53, 26 September 2022

mSarlet-I

Usage and Biology

We conducted a simple test to see if our design met the expection.
We designed the genetic circuit that contains sulAp (sulA promoter), Amp30E, relE gene (RelE toxin), mScarlet-I and 3WJ-Bro(3WJ-Bro) , and transferred it into DH10B competent cells (The control group did not contain the Amp30E Amplification Device).
The relE toxin gene and the mScarlet-I gene were fused to construct a fusion gene, in order to detect the amount of relE protein synthesis. We removed the terminator of relE and fused it to the mScarlet-I gene.

Sequence and Features

Assembly Compatibility:

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 ==Characterized by CAFA_China 2022==
 *We conducted a simple test to see if our design met the expection.
-*We designed the genetic circuit that contains sulAp ([[Part:BBa_K518010|sulA promoter]]), Amp30E, relE gene ([[Part:BBa_K185047|RelE toxin]]), mScarlet-I ([[Part:BBa_K3977002|mSarlet-I]]) and 3WJ-Bro([[Part:BBa K4226000 |3WJ-Bro]]) , and transferred it into DH10B competent cells (The control group did not contain the Amp30E Amplification Device).
+*We designed the genetic circuit that contains sulAp ([[Part:BBa_K518010|sulA promoter]]), Amp30E, relE gene ([[Part:BBa_K185047|RelE toxin]]), mScarlet-I and 3WJ-Bro([[Part:BBa K4226000 |3WJ-Bro]]) , and transferred it into DH10B competent cells (The control group did not contain the Amp30E Amplification Device).
 *The relE toxin gene and the mScarlet-I gene were fused to construct a fusion gene, in order to detect the amount of relE protein synthesis. We removed the terminator of relE and fused it to the mScarlet-I gene.