Difference between revisions of "Part:BBa K3977002"
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==Characterized by CAFA_China 2022== | ==Characterized by CAFA_China 2022== | ||
*We conducted a simple test to see if our design met the expection. | *We conducted a simple test to see if our design met the expection. | ||
| − | *We designed the genetic circuit that contains sulAp ([[Part:BBa_K518010|sulA promoter]]), Amp30E, relE gene ([[Part:BBa_K185047|RelE toxin]]), mScarlet-I ([[Part:BBa_K3977002|mSarlet-I]]) and 3WJ-Bro, and transferred it into DH10B competent cells (The control group did not contain the Amp30E Amplification Device). | + | *We designed the genetic circuit that contains sulAp ([[Part:BBa_K518010|sulA promoter]]), Amp30E, relE gene ([[Part:BBa_K185047|RelE toxin]]), mScarlet-I ([[Part:BBa_K3977002|mSarlet-I]]) and 3WJ-Bro([[Part:BBa K4226000 |3WJ-Bro]]) , and transferred it into DH10B competent cells (The control group did not contain the Amp30E Amplification Device). |
*The relE toxin gene and the mScarlet-I gene were fused to construct a fusion gene, in order to detect the amount of relE protein synthesis. We removed the terminator of relE and fused it to the mScarlet-I gene. | *The relE toxin gene and the mScarlet-I gene were fused to construct a fusion gene, in order to detect the amount of relE protein synthesis. We removed the terminator of relE and fused it to the mScarlet-I gene. | ||
Revision as of 06:53, 26 September 2022
mSarlet-I
mSarlet-I
Usage and Biology
Characterized by CAFA_China 2022
- We conducted a simple test to see if our design met the expection.
- We designed the genetic circuit that contains sulAp (sulA promoter), Amp30E, relE gene (RelE toxin), mScarlet-I (mSarlet-I) and 3WJ-Bro(3WJ-Bro) , and transferred it into DH10B competent cells (The control group did not contain the Amp30E Amplification Device).
- The relE toxin gene and the mScarlet-I gene were fused to construct a fusion gene, in order to detect the amount of relE protein synthesis. We removed the terminator of relE and fused it to the mScarlet-I gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 667
- 1000COMPATIBLE WITH RFC[1000]