Difference between revisions of "Part:BBa K4226000"

Revision as of 05:43, 26 September 2022

3WJ-Bro

3WJ-Bro is used as a protein- independent reporter system based on a fluorescent RNA aptamer. 3WJ-Bro is applied as a gene marker in creating a system for reporting the presence and expression of target gene, which is relE toxin gene at the transcriptional level in the project of CAFA_China (2022). The 3WJ-Bro can be used to ligate fluorescent aptamers to examine the relE RNA at transcriptional level in Escherichia coli cells, obviating the need for accumulation of foreign proteins.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Characterized by CAFA_China 2022

We measured the OD600 and fluorescence values under 485/510nm. Then, the fluorescence per OD was calculated.
According to the results, the 3WJ-Bro can be used to ligate fluorescent aptamers to examine the relE RNA at transcriptional level.

Visual Results as Normally Open Switches

@@ Line 13: / Line 13: @@
 == Characterized by CAFA_China 2022 ==
-*According to the results, the reporter system includes 3WJ-Bro and mScarlet could successfully reflect the expression level of relE protein.
 *We measured the OD600 and fluorescence values under 485/510nm. Then, the fluorescence per OD was calculated. <br>
+*According to the results, the 3WJ-Bro can be used to ligate fluorescent aptamers to examine the relE RNA at transcriptional level.
 [[File:3WJ-Bro.png|600px|thumb|center|Visual Results as Normally Open Switches]]<br>