Difference between revisions of "Part:BBa K4226000"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | [[File:3WJ-Bro.png|600px|thumb|center|3WJ-Bro was ligated with fluorescent aptamers in order to examine the level of relE RNA synthesis (485/510nm).]] | + | [[File:3WJ-Bro.png|600px|thumb|center|3WJ-Bro was ligated with fluorescent aptamers in order to examine the level of relE RNA synthesis (485/510nm).]]<br> |
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Revision as of 03:43, 26 September 2022
3WJ-Bro
3WJ-Bro is used as a protein- independent reporter system based on a fluorescent RNA aptamer. 3WJ-Bro is applied as a gene marker in creating a system for reporting the presence and expression of target gene, which is relE toxin gene at the transcriptional level in the project of CAFA_China (2022). The 3WJ-Bro can be used to ligate fluorescent aptamers to examine the relE RNA at transcriptional level in Escherichia coli cells, obviating the need for accumulation of foreign proteins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]