Difference between revisions of "Part:BBa K4268000:Experience"

 
(→‎Applications of BBa_K4268000)
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===Applications of BBa_K4268000===
 
===Applications of BBa_K4268000===
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With the generosity of IDT, SUNY_Oneonta iGEM synthesized the Head-Tail Connector part. This part is RCF1000 compatible and contains SapI sites at either end of the sequence. The part was resuspended, restriction digested, and ligated into PSB1C00. The complex was then transformed into DH5&a cells and was left to grow overnight. After white colony growth was discovered in the transformed cells, five white colonies underwent a Colony PCR to determine if the desired insert was absorbed. 
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Insert gel figure
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Caption: Colony PCR of five colonies suspected of coating the insert and one colony containing an RFP.
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At about 2000bp the gel indicates that all five colonies contain the insert. Thus
  
 
===User Reviews===
 
===User Reviews===

Revision as of 23:22, 25 September 2022


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4268000

With the generosity of IDT, SUNY_Oneonta iGEM synthesized the Head-Tail Connector part. This part is RCF1000 compatible and contains SapI sites at either end of the sequence. The part was resuspended, restriction digested, and ligated into PSB1C00. The complex was then transformed into DH5&a cells and was left to grow overnight. After white colony growth was discovered in the transformed cells, five white colonies underwent a Colony PCR to determine if the desired insert was absorbed.

Insert gel figure Caption: Colony PCR of five colonies suspected of coating the insert and one colony containing an RFP.

At about 2000bp the gel indicates that all five colonies contain the insert. Thus

User Reviews

UNIQ9ea0d062612832e9-partinfo-00000000-QINU UNIQ9ea0d062612832e9-partinfo-00000001-QINU