Difference between revisions of "Part:BBa K182001:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K182001=== | ===Applications of BBa_K182001=== | ||
| + | <!-- Template for a user review | ||
| + | {|width='80%' style='border:1px solid gray' | ||
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| + | |width='10%'| | ||
| + | <partinfo>BBa_k182001AddReview number</partinfo> | ||
| + | <I>Username</I> | ||
| + | |width='60%' valign='top'| | ||
| + | Enter the review inofrmation here. | ||
| + | |}; | ||
| + | <!-- End of the user review template --> | ||
| + | {|width='80%' style='border:1px solid gray' | ||
| + | |- | ||
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| + | <partinfo>BBa_k182001 AddReview 5</partinfo> | ||
| + | <I>Aberdeen_Scotland 2009</I> | ||
| + | |width='60%' valign='top'| | ||
| + | The transformation, miniprep and the gel (undigested and digested with the XbaI and pstI) worked. Further tests were confirmed by PCR analysis and subsequent sequencing. The construct can further be characterised with the combination of BBa_I13601 which contians a LacI operator with a CFP reporter. The combination of BBa_K182001 and BBa_I13601 should repress the CFP expression. This inhibition can be removed by the addition of IPTG which will restore the CFP expression in the cell. | ||
| + | |} | ||
| + | <!-- End of the user review template --> | ||
| + | <!-- DON'T DELETE --><partinfo>BBa_k182001 EndReviews</partinfo> | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 09:26, 11 August 2009
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K182001
|
•••••
Aberdeen_Scotland 2009 |
The transformation, miniprep and the gel (undigested and digested with the XbaI and pstI) worked. Further tests were confirmed by PCR analysis and subsequent sequencing. The construct can further be characterised with the combination of BBa_I13601 which contians a LacI operator with a CFP reporter. The combination of BBa_K182001 and BBa_I13601 should repress the CFP expression. This inhibition can be removed by the addition of IPTG which will restore the CFP expression in the cell. |
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User Reviews
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