Difference between revisions of "Part:BBa K4202004:Experience"
(→‎Expression of BBa_K4202004)
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===Expression of BBa_K4202004===
 
===Expression of BBa_K4202004===
  
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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After chemical transformation of plasmid with this part, the transformed <i>Bacillus subtilis</i> WB600 were cultured in optimized LB and SMM medium, and obtained crude enzyme solution by centrifugation and ultrasonic disruption.Then we detected the molecular mass by SDS-PAGE and coomassie blue staining.
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how you used this part and how it worked out.
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SDS-PAGE displayed bands of 37kDa and 74kDa for CA monomer and dimer, which didn' t exist in the control group(Fig.1-1)
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===Applications of BBa_K4202004===
 
===Applications of BBa_K4202004===

Revision as of 13:37, 25 September 2022


Expression of BBa_K4202004

After chemical transformation of plasmid with this part, the transformed Bacillus subtilis WB600 were cultured in optimized LB and SMM medium, and obtained crude enzyme solution by centrifugation and ultrasonic disruption.Then we detected the molecular mass by SDS-PAGE and coomassie blue staining.
SDS-PAGE displayed bands of 37kDa and 74kDa for CA monomer and dimer, which didn' t exist in the control group(Fig.1-1)

Applications of BBa_K4202004

User Reviews

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