Difference between revisions of "Part:BBa K4463002"
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CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of SmtA, a protein belongs to Metallothionein family, achieved by linking to csgA. SUMO tag was added to improve the solubility of recombinant protein, csgA and SmtA. Improved parts is based on part <a href="http://https://parts.igem.org/Part:BBa_K519010">BBa_K519010</a>.
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CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of SmtA, a protein belongs to Metallothionein family, achieved by linking to csgA. SUMO tag was added to improve the solubility of recombinant protein, csgA and SmtA. Improved parts is based on part <a href="https://parts.igem.org/Part:BBa_K519010">BBa_K519010</a>.
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Revision as of 11:34, 25 September 2022


SUMO-CsgA-SmtA
CsgA is the major curli subunit protein of E.coli. Therefor, the surface display of SmtA, a protein belongs to Metallothionein family, achieved by linking to csgA. SUMO tag was added to improve the solubility of recombinant protein, csgA and SmtA. Improved parts is based on part BBa_K519010.  


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 863
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 863
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 297
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 863
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 863
    Illegal AgeI site found at 891
  • 1000
    COMPATIBLE WITH RFC[1000]