Difference between revisions of "Part:BBa K4158012"
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<partinfo>BBa_K4158012 short</partinfo>
 
<partinfo>BBa_K4158012 short</partinfo>
  
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RBS and coding site for the SRTF1　protein with TEV site and His tag. edcR binds to edcR regulator and works as repressor. 17β-estradiol((17β)-estra-1,3,5(10)-triene-3,17-diol) binds to edcR and inhibit its operation, therefore promoting transcription.
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RBS and coding site for the SRTF1　protein with TEV site and His tag. SRTF1 binds to SRTF1 regulator and works as repressor. Progesterone((1S,3aS,3bS,9aR,9bS,11aS)-1-Acetyl-9a,11a-dimethyl-1,2,3,3a,3b,4,5,8,9,9a,9b,10,11,11a-tetradecahydro-7H-cyclopenta[a]phenanthren-7-one) binds to SRTF1 and inhibit its operation, therefore promoting transcription.
  
 
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Revision as of 07:28, 25 September 2022


SRTF1(E coli Codon Optimized sequence)

RBS and coding site for the SRTF1　protein with TEV site and His tag. SRTF1 binds to SRTF1 regulator and works as repressor. Progesterone((1S,3aS,3bS,9aR,9bS,11aS)-1-Acetyl-9a,11a-dimethyl-1,2,3,3a,3b,4,5,8,9,9a,9b,10,11,11a-tetradecahydro-7H-cyclopenta[a]phenanthren-7-one) binds to SRTF1 and inhibit its operation, therefore promoting transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 152
    Illegal PstI site found at 488
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 152
    Illegal PstI site found at 488
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 466
    Illegal BamHI site found at 532
    Illegal BamHI site found at 683
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 152
    Illegal PstI site found at 488
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 152
    Illegal PstI site found at 488
    Illegal AgeI site found at 173
    Illegal AgeI site found at 458
  • 1000
    COMPATIBLE WITH RFC[1000]