Difference between revisions of "Part:BBa K4158009"
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<partinfo>BBa_K4158009 short</partinfo> | <partinfo>BBa_K4158009 short</partinfo> | ||
− | RBS and coding site for the edcR protein with TEV site and His tag. edcR binds to edcR regulator and works as repressor. 17β-estradiol binds to edcR and inhibit its operation, therefore promoting transcription. | + | RBS and coding site for the edcR protein with TEV site and His tag. edcR binds to edcR regulator and works as repressor. 17β-estradiol((17β)-estra-1,3,5(10)-triene-3,17-diol) binds to edcR and inhibit its operation, therefore promoting transcription. |
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Revision as of 07:25, 25 September 2022
RBS-edcR
RBS and coding site for the edcR protein with TEV site and His tag. edcR binds to edcR regulator and works as repressor. 17β-estradiol((17β)-estra-1,3,5(10)-triene-3,17-diol) binds to edcR and inhibit its operation, therefore promoting transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 750
Illegal XhoI site found at 38 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 308
Illegal AgeI site found at 318 - 1000COMPATIBLE WITH RFC[1000]