Difference between revisions of "Part:BBa K4380000"
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| − | + | Carbohydrate-binding module of family 3 (CBM3) or cellulose binding domain is a protein that has the ability to robustly bind cellulose. The protein coded from this sequence comes from a thermophilic, anaerobic bacterium Clostridium thermocellum. The part can be succesfully used in safety level 1 laboratory for different, but useful reasons. The CBM3 is overexpressed in Escherichia coli and it is possible to take advantage of its affinity properties to purify recombinant proteins on cellulose fibers, (reducing significantly the costs of purification), to imobilize proteins on a cellulose membrane as well as a a purification tag for antimicrobial peptides. . CBM3 proteins can not only bind to crystalline cellulose, but can also interact with smaller affinity (~500 lower) to chitin and xyloglucan. | |
Revision as of 22:09, 24 September 2022
Carbohydrate-binding module of family 3 (CBM3) or cellulose binding domain is a protein that has the ability to robustly bind cellulose. The protein coded from this sequence comes from a thermophilic, anaerobic bacterium Clostridium thermocellum. The part can be succesfully used in safety level 1 laboratory for different, but useful reasons. The CBM3 is overexpressed in Escherichia coli and it is possible to take advantage of its affinity properties to purify recombinant proteins on cellulose fibers, (reducing significantly the costs of purification), to imobilize proteins on a cellulose membrane as well as a a purification tag for antimicrobial peptides. . CBM3 proteins can not only bind to crystalline cellulose, but can also interact with smaller affinity (~500 lower) to chitin and xyloglucan.