Difference between revisions of "Part:BBa K4241012"
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https://www.nature.com/articles/s42003-020-0939-8 | https://www.nature.com/articles/s42003-020-0939-8 | ||
This is a enhanced RBS/start codon region which was proven to yield higher protein expression in bacterial chassis. | This is a enhanced RBS/start codon region which was proven to yield higher protein expression in bacterial chassis. | ||
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| + | This will initiate the protein translation at the start codon including a Met residue. Like any other pET plasmid expression chassis, it would have this scar. | ||
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Revision as of 14:50, 24 September 2022
TIR-2 translation initiation region 2
https://www.nature.com/articles/s42003-020-0939-8 This is a enhanced RBS/start codon region which was proven to yield higher protein expression in bacterial chassis.
This will initiate the protein translation at the start codon including a Met residue. Like any other pET plasmid expression chassis, it would have this scar.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]