Difference between revisions of "Part:BBa K4158010:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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1. Prepare pACYC184 cloning vector and this part as BioBrick.
 
1. Prepare pACYC184 cloning vector and this part as BioBrick.
  
−
2. Restriction and insertion cloning with pACYC184 and this BioBrick productions and restriction enzymes HindIII and BamHI."
+
2. Restriction and insertion cloning with pACYC184 and this BioBrick productions and restriction enzymes HindIII and BamHI.
  
 
===Source===
 
===Source===

Latest revision as of 02:47, 24 September 2022


Psrtf1-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 13
    Illegal NheI site found at 36
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 826
    Illegal XhoI site found at 529
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

If you want to get the same plasmid that we experimented with, construct the plasmid along the way below.

1. Prepare pACYC184 cloning vector and this part as BioBrick.

2. Restriction and insertion cloning with pACYC184 and this BioBrick productions and restriction enzymes HindIII and BamHI.

Source

test

References