Difference between revisions of "Part:BBa K4158009:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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1. Prepare pET26b(+) cloning vector and this part as BioBrick.
 
1. Prepare pET26b(+) cloning vector and this part as BioBrick.
  
−
2. Restriction and insertion cloning with pJL1 and this BioBrick productions and restriction enzymes XbaI and BamHI."
+
2. Restriction and insertion cloning with pET26b(+) and this BioBrick productions and restriction enzymes XbaI and BamHI."
  
 
===Source===
 
===Source===

Latest revision as of 02:22, 24 September 2022


RBS-edcR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 750
    Illegal XhoI site found at 38
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 308
    Illegal AgeI site found at 318
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

"If you want to get the same plasmid that we experimented with, construct the plasmid along the way below.

1. Prepare pET26b(+) cloning vector and this part as BioBrick.

2. Restriction and insertion cloning with pET26b(+) and this BioBrick productions and restriction enzymes XbaI and BamHI."

Source

test

References