Difference between revisions of "Part:BBa K4268000:Design"
Jazmine124 (Talk | contribs) (→Design Notes) |
Jazmine124 (Talk | contribs) (→Design Notes) |
||
| Line 9: | Line 9: | ||
To determine which sequences would be used to code for the shell of the phage, a series of comparisons were made. SUNY_Oneonta iGEM had identified T7 and T4-like phages. The team chose to utilize T7-like phages due to their smaller genome compared to T4-like phages. The smaller genome would allow greater efficiency and ease in cloning the chosen species' genes. | To determine which sequences would be used to code for the shell of the phage, a series of comparisons were made. SUNY_Oneonta iGEM had identified T7 and T4-like phages. The team chose to utilize T7-like phages due to their smaller genome compared to T4-like phages. The smaller genome would allow greater efficiency and ease in cloning the chosen species' genes. | ||
| − | Synechococcus sp PCC 11901, was decided as the desired cyanobacterial species that SUNY_Oneonta iGEM would like to focus on? S-TIP 37 is the infecting cyanophage of this cyanobacterial species. However, the genome has not undergone full sequencing and many of its genes remain hypothetical proteins. Thus, comparison to Syn-5, another T7-like phage that has been fully sequenced, was needed. Both S-TIP 37 and Syn-5 are structurally and genetically similar allowing the team to distinguish the genes needed for producing the phage shell. | + | "Synechococcus sp PCC 11901", was decided as the desired cyanobacterial species that SUNY_Oneonta iGEM would like to focus on? S-TIP 37 is the infecting cyanophage of this cyanobacterial species. However, the genome has not undergone full sequencing and many of its genes remain hypothetical proteins. Thus, comparison to Syn-5, another T7-like phage that has been fully sequenced, was needed. Both S-TIP 37 and Syn-5 are structurally and genetically similar allowing the team to distinguish the genes needed for producing the phage shell. |
Insert poster table comparison | Insert poster table comparison | ||
| − | + | --- | |
Upon the isolation of the Head-Tail Connector sequence, the following edits were made: | Upon the isolation of the Head-Tail Connector sequence, the following edits were made: | ||
Revision as of 11:21, 23 September 2022
S-TIP37 Head-Tail Connector
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 346
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To determine which sequences would be used to code for the shell of the phage, a series of comparisons were made. SUNY_Oneonta iGEM had identified T7 and T4-like phages. The team chose to utilize T7-like phages due to their smaller genome compared to T4-like phages. The smaller genome would allow greater efficiency and ease in cloning the chosen species' genes.
"Synechococcus sp PCC 11901", was decided as the desired cyanobacterial species that SUNY_Oneonta iGEM would like to focus on? S-TIP 37 is the infecting cyanophage of this cyanobacterial species. However, the genome has not undergone full sequencing and many of its genes remain hypothetical proteins. Thus, comparison to Syn-5, another T7-like phage that has been fully sequenced, was needed. Both S-TIP 37 and Syn-5 are structurally and genetically similar allowing the team to distinguish the genes needed for producing the phage shell.
Insert poster table comparison
--- Upon the isolation of the Head-Tail Connector sequence, the following edits were made:
- Restriction sites were removed/altered to ensure RCF10 and RCF1000 compatibility
- To complete Level 0 Golden Gate Assembly, the sequence contains SapI sites at the ends
Source
The source of this sequence is from the S-TIP37 genome (GenBanK: NC_048026.1)
References
The S-TIP37 genome was found on GenomeNet