Difference between revisions of "Part:BBa K4235016"

Revision as of 23:49, 22 September 2022

pET His6 LIC cloning vector (2Bc-T)

The Human Protein S gene PROS1 () was inserted into this LIC cloning vector and was transformed into E.coli strains Bl21 and origami B for expression.

This vector contains the following parts:

T7 Promoter: BBa_K4235012
RBS: BBa_K4235014
Ampicillin resistance cassette (AmpR promoter+CDS): BBa_K4235029
T7 terminator: BBa_K4235013

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 4105
Illegal PstI site found at 750
Illegal PstI site found at 4090
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 4543
Illegal PstI site found at 750
Illegal PstI site found at 4090
Illegal NotI site found at 4081
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3990
Illegal BamHI site found at 4207
Illegal XhoI site found at 4098
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 4105
Illegal PstI site found at 750
Illegal PstI site found at 4090
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 4105
Illegal PstI site found at 750
Illegal PstI site found at 4090
Illegal NgoMIV site found at 3076
Illegal NgoMIV site found at 3430
Illegal NgoMIV site found at 3590
Illegal NgoMIV site found at 3958
Illegal NgoMIV site found at 4054
Illegal NgoMIV site found at 4237
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 924
Illegal BsaI.rc site found at 4029
Illegal SapI.rc site found at 2006

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K4235016 short</partinfo>
+[[Image:Vector_sbu.jpg|700px|center|]]
 The Human Protein S gene PROS1 () was inserted into this LIC cloning vector and was transformed into '''E.coli''' strains Bl21 and origami B for expression.