Difference between revisions of "Part:BBa K4361317"

 
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<ul>
  
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<li>.</li>
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<li>C 497 > T, resulting in substitution S166L</li>
 +
<li>G 628 > A, resulting in substitution D210N</li>
 
</ul>
 
</ul>
  

Revision as of 14:38, 22 September 2022


BlcR V68K

A mutant of the BlcR protein, created through site-directed mutagenesis with primers R3 (Part:BBa_K4361218) and F3.8 V68K (Part:BBa_K4361226). For this mutant, the valine in position 68 has been changed to lysine by mutating the GTG codon to AAA.

This mutant also contains the following nucleotide mutations outside of the targeted site:

  • C 497 > T, resulting in substitution S166L
  • G 628 > A, resulting in substitution D210N

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 589