Difference between revisions of "Part:BBa K4361302"
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<ul>
 
<ul>
  
−
<li>.</li>
+
<li>G 474 > T, silent mutation</li>
 +
<li>T 529 > C, resulting in substitution F177L</li>
 +
<li>G 606 > T, silent mutation</li>
 +
<li>T 618 > A, silent mutation</li>
 +
<li>G 648 > T, resulting in substitution E216D</li>
 +
<li>T 681 > A, resulting in substitution E227D</li>
 +
<li>G 742 > A, resulting in substitution E248A</li>
 +
<li>A 745 > C, resulting in substitution K249Q</li>
 
</ul>
 
</ul>
  

Revision as of 12:58, 22 September 2022


BlcR A40V

A mutant of the BlcR protein, created through site-directed mutagenesis with primers R4 (Part:BBa_K4361204) and F4.1 A40V (Part:BBa_K4361205). For this mutant, the alanine in position 40 has been changed to valine by mutating the GCG codon to GTG.

This mutant also contains the following nucleotide mutations outside of the targeted site:

  • G 474 > T, silent mutation
  • T 529 > C, resulting in substitution F177L
  • G 606 > T, silent mutation
  • T 618 > A, silent mutation
  • G 648 > T, resulting in substitution E216D
  • T 681 > A, resulting in substitution E227D
  • G 742 > A, resulting in substitution E248A
  • A 745 > C, resulting in substitution K249Q

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 589