Difference between revisions of "Part:BBa K4361301"
Line 9: Line 9:
  
 
<li>A 50 > C, silent mutation</li>
 
<li>A 50 > C, silent mutation</li>
 +
<li>G 166 > T, resulting in substitution D56Y</li>
 +
<li>G 237 > A, silent mutation</li>
 +
<li>A 341 > C, resulting in substitution H114P</li>
 +
<li>G 404 > T, resulting in substitution G135V</li>
 +
<li>G 416 > T, resulting in substitution S139I</li>
 
</ul>
 
</ul>
  

Revision as of 12:41, 22 September 2022


BlcR D37V

A mutant of the BlcR protein, created through site-directed mutagenesis with primers R1 (Part:BBa_K4361200) and F1.2 D37V (Part:BBa_K4361202). For this mutant, the aspartic acid in position 37 has been changed to valine by mutating the GAC codon to GTG.

This mutant also contains the following nucleotide mutations outside of the targeted site:

  • A 50 > C, silent mutation
  • G 166 > T, resulting in substitution D56Y
  • G 237 > A, silent mutation
  • A 341 > C, resulting in substitution H114P
  • G 404 > T, resulting in substitution G135V
  • G 416 > T, resulting in substitution S139I

Sequencing data only contained this part's sequence up until and including nucleotide 421. All nucleotides after are presumed to be identical to those found in the original codon optimized BlcR, Part:BBa_K4361100.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 694
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 589