Difference between revisions of "Part:BBa R0051:Experience"
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Transformation and o/n culture is Okay, but we couldn't extract plasmid by miniprep. We couldn't detect band by Electrophoresis. We don't know why, but this occured 2 times. | Transformation and o/n culture is Okay, but we couldn't extract plasmid by miniprep. We couldn't detect band by Electrophoresis. We don't know why, but this occured 2 times. | ||
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+ | <!-- Aberdeen_Scotland 2009 user review --> | ||
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+ | <partinfo>BBa_R0051 AddReview 3</partinfo> | ||
+ | <I> Aberdeen_Scotland 2009 </I> | ||
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The initial gel worked although the obvious confirmation was not possible due to the very small fragment size (49bp).But later it was confirmed following its usage by forming the part BBa_K182002 through the PCR gel analysis and the sequencing. | The initial gel worked although the obvious confirmation was not possible due to the very small fragment size (49bp).But later it was confirmed following its usage by forming the part BBa_K182002 through the PCR gel analysis and the sequencing. | ||
Revision as of 09:59, 7 August 2009
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_R0051
User Reviews
UNIQ518d48f8913d5933-partinfo-00000000-QINU
•••••
Antiquity |
This review comes from the old result system and indicates that this part worked in some test. |
•
Tokyo_Tech |
Transformation and o/n culture is Okay, but we couldn't extract plasmid by miniprep. We couldn't detect band by Electrophoresis. We don't know why, but this occured 2 times. |
•••
Aberdeen_Scotland 2009 |
The initial gel worked although the obvious confirmation was not possible due to the very small fragment size (49bp).But later it was confirmed following its usage by forming the part BBa_K182002 through the PCR gel analysis and the sequencing.
|