Difference between revisions of "Part:BBa K4150004"
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<span style="color:#00000000">This</span>The chicken ovalbumin sequence obtained from KEGG database ([https://www.genome.jp/entry/gga:396058 Gallus gallus]) was codon optimzed for expression in E. coli and synthesized by Integrated DNA Technologies, Inc. with a connection of a His tag and a GS linker at N-terminus. The ovalbumin (OVA) is a glycoprotein, which is a major component of chicken egg whites, and harbors immunogenic properties in vaccination experiments<ref>Geary TW, Reeves JJ. Production of a genetically engineered inhibin vaccine. Vaccine. 1996 Sep;14(13):1273-9. doi: 10.1016/s0264-410x(96)00014-x. PMID: 8961517.</ref>. The recombinant OVA protein is readily purified in E. coli BL21 system driven by T7 promoter and triggered by IPTG induction.     
 
<span style="color:#00000000">This</span>The chicken ovalbumin sequence obtained from KEGG database ([https://www.genome.jp/entry/gga:396058 Gallus gallus]) was codon optimzed for expression in E. coli and synthesized by Integrated DNA Technologies, Inc. with a connection of a His tag and a GS linker at N-terminus. The ovalbumin (OVA) is a glycoprotein, which is a major component of chicken egg whites, and harbors immunogenic properties in vaccination experiments<ref>Geary TW, Reeves JJ. Production of a genetically engineered inhibin vaccine. Vaccine. 1996 Sep;14(13):1273-9. doi: 10.1016/s0264-410x(96)00014-x. PMID: 8961517.</ref>. The recombinant OVA protein is readily purified in E. coli BL21 system driven by T7 promoter and triggered by IPTG induction.     
  
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===Characterization===
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===Purification===
  
  

Revision as of 05:08, 20 September 2022


His-OVA

ThisThe chicken ovalbumin sequence obtained from KEGG database (Gallus gallus) was codon optimzed for expression in E. coli and synthesized by Integrated DNA Technologies, Inc. with a connection of a His tag and a GS linker at N-terminus. The ovalbumin (OVA) is a glycoprotein, which is a major component of chicken egg whites, and harbors immunogenic properties in vaccination experiments[1]. The recombinant OVA protein is readily purified in E. coli BL21 system driven by T7 promoter and triggered by IPTG induction.

Characterization



Purification



Reference

  1. ↑ Geary TW, Reeves JJ. Production of a genetically engineered inhibin vaccine. Vaccine. 1996 Sep;14(13):1273-9. doi: 10.1016/s0264-410x(96)00014-x. PMID: 8961517.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 373