Difference between revisions of "Part:BBa K4158007:Design"

 
(Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
test
+
If you want to get same plasmid that we experimented with, construct the plasmid along this way below.
  
 +
1. Prepare pJL1 cloning vector (addgene Plasmid #69496)and this part as BioBrick.
  
 +
2. Restriction and insertion cloning with pJL1 and this BioBrick productions and restriction enzymes XbaI and salI.
  
 
===Source===
 
===Source===

Revision as of 09:23, 18 September 2022


RBS-AtzC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1133
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1133
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1133
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1133
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1133
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

If you want to get same plasmid that we experimented with, construct the plasmid along this way below.

1. Prepare pJL1 cloning vector (addgene Plasmid #69496)and this part as BioBrick.

2. Restriction and insertion cloning with pJL1 and this BioBrick productions and restriction enzymes XbaI and salI.

Source

test

References