Difference between revisions of "Part:BBa K4348002:Design"

 
 
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===Design Notes===
 
===Design Notes===
−
Codon optimized for expression in E. coli
+
Codon optimized for expression in E. coli. We used NcoI and NotI to clone this gene instead of EcoRI and PstI, because it contains an EcoRI site in the coding sequence.
  
  

Latest revision as of 00:50, 17 September 2022


AKR1C4_his


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 315
    Illegal NgoMIV site found at 780
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 403


Design Notes

Codon optimized for expression in E. coli. We used NcoI and NotI to clone this gene instead of EcoRI and PstI, because it contains an EcoRI site in the coding sequence.


Source

Homo sapiens

References