Difference between revisions of "Part:BBa K274001:Design"

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===Source===
 
===Source===
  
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The melA gene comes from the Rhizobium etli CFN 42 symbiotic plasmid p42d (accession number U80928). The origonal plasmid was created by Christine Nicole S. Santos and Gregory Stephanopoulos (reference: http://aem.asm.org/cgi/content/abstract/74/4/1190)
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The melA gene comes from the Rhizobium etli CFN 42 symbiotic plasmid p42d (accession number U80928). The original plasmid was created by Christine Nicole S. Santos and Gregory Stephanopoulos.
  
 
===References===
 
===References===
  
 
Christine Nicole S. Santos and Gregory Stephanopoulos ''Melanin-Based High-Throughput Screen for L-Tyrosine Production in Escherichia coli'' American Society for Microbiology 2007 74(4) 1190-1197 doi:10.1128/AEM.02448-07 [http://aem.asm.org/cgi/content/abstract/74/4/1190 link title]
 
Christine Nicole S. Santos and Gregory Stephanopoulos ''Melanin-Based High-Throughput Screen for L-Tyrosine Production in Escherichia coli'' American Society for Microbiology 2007 74(4) 1190-1197 doi:10.1128/AEM.02448-07 [http://aem.asm.org/cgi/content/abstract/74/4/1190 link title]

Revision as of 15:48, 4 August 2009

melanin pigment


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1332
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1184
    Illegal BsaI.rc site found at 489


Design Notes

Created by PCR

The only changes to the sequence from the plasmid kindly provided by The Massachusetts Institute of Technology and Gregory Stephanopoulos was the removal of two PstI restriction sites. This was done by converting CAG to CAA in the second codon of the site.

Source

The melA gene comes from the Rhizobium etli CFN 42 symbiotic plasmid p42d (accession number U80928). The original plasmid was created by Christine Nicole S. Santos and Gregory Stephanopoulos.

References

Christine Nicole S. Santos and Gregory Stephanopoulos Melanin-Based High-Throughput Screen for L-Tyrosine Production in Escherichia coli American Society for Microbiology 2007 74(4) 1190-1197 doi:10.1128/AEM.02448-07 [http://aem.asm.org/cgi/content/abstract/74/4/1190 link title]