Difference between revisions of "Part:BBa K238013"

Revision as of 13:40, 2 August 2009

Exact promoter of the blue light receptor

This is a promoter which normally lies before the ycgZ gene, a part of an operon consisting of several genes used by E.coli for regulating the structure of their biofilm. It is regualted by the ycgF/E system. This ssytem has a receptor, ycgF, which is responsive of blue light. when blue light strikes, the receptor changes conformation and dimerizes. This allows it to bind to the ycgE repressor through its EAL-domain releasing the repressor from the promoter region. the inverted repeats shown here is the DNA we expect ycgE to bind to when repressing the promoter. For more info: Natalia Tschowri, Susan Busse and Regine Hengge, The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli. Genes and development23: 522-534 (2009)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K238013 short</partinfo>
-ycgF/E regulated promoter region ycgf is a cytoplasmic receptor which responds to blue light. it contains a Blue light Using FAD-Domain (BLUF) and an EAL domain. when blue light strikes, the receptor changes conformation and dimerizes. This allows it to bind to ycgE repressor through its EAL-domain releasing the repressor from this promoter region. See reference for more info: Natalia Tschowri, Susan Busse and Regine Hengge, The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli. Genes and development23: 522-534 (2009)
+This is a promoter which normally lies before the ycgZ gene, a part of an operon consisting of several genes used by E.coli for regulating the structure of their biofilm. It is regualted by the ycgF/E system. This ssytem has a receptor, ycgF, which is responsive of blue light. when blue light strikes, the receptor changes conformation and dimerizes. This allows it to bind to the ycgE repressor through its EAL-domain releasing the repressor from the promoter region. the inverted repeats shown here is the DNA we expect ycgE to bind to when repressing the promoter.
+For more info: Natalia Tschowri, Susan Busse and Regine Hengge, The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli. Genes and development23: 522-534 (2009)