Difference between revisions of "Part:BBa K259000:Design"
(→‎Construction)
 
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This part was constructed via PCR amplification;
 
This part was constructed via PCR amplification;
−
**Fwd Primer: 5'-GCTCTAGATGGCGCGTTCCAAAACTGCTG-3'
 
−
**Rev Primer: 5'-GCACTAGTATTATTAGAAACGGAAGGTTGC-3'
 
−
 
  
 +
  Fwd Primer: 5'-GCTCTAGATGGCGCGTTCCAAAACTGCTG-3'
 +
  Rev Primer: 5'-GCACTAGTATTATTAGAAACGGAAGGTTGC-3'
  
 
===Design Notes===
 
===Design Notes===

Latest revision as of 11:08, 2 August 2009

fhuA - Outer membrane transporter for ferrichrome-iron


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 682
    Illegal AgeI site found at 820
    Illegal AgeI site found at 1225
    Illegal AgeI site found at 1999
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 724

Construction

This part was constructed via PCR amplification; 

 Fwd Primer: 5'-GCTCTAGATGGCGCGTTCCAAAACTGCTG-3'
 Rev Primer: 5'-GCACTAGTATTATTAGAAACGGAAGGTTGC-3'

Design Notes

The coding sequence (CDS) was already in compliance with assembly standard 10 (RFC10).


Source

fhuA is part of the chromosomal genome of E.coli. In particular it can be found at 3.61min and follows the clockwise direction.


References

Locher K.P., Rees B., Koebnik R., Mitschler A., Moulinier L., Rosenusch J.P. and Moras D. (1998),Transmembrane signalling across the Ligand-Gated FhuAReceptor: Crystal structures of Free and Ferrichrome-Bound States reveal allosteric changes. Cell 95,771-778.

[http://www.uniprot.org/uniprot/P06971 UniProt access number P06971]

[http://ecogene.org/geneInfo.php?eg_id=EG10302 EcoGene Database access number EG10302]