Difference between revisions of "Part:BBa K175003"
 
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<partinfo>BBa_K175003 short</partinfo>
 
<partinfo>BBa_K175003 short</partinfo>
  
−
Restriction site for the homing endonuclease I-SceI.
+
This knockout of the entry exclusion membrane bound protein allows cells containing R751 to conjugate with each other.
  
−
Sequence will be cut as follows:
 
  
−
5' A G T T A C G C T A G G G A T A A^C A G G G T A A T A T A G 3'<br>
 
−
3' T C A A T G C G A T C C C^T A T T G T C C C A T T A T A T C 5'
 
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 09:55, 1 August 2009

R751 plasmid with trbK knockout

This knockout of the entry exclusion membrane bound protein allows cells containing R751 to conjugate with each other.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]