Difference between revisions of "Part:BBa K175027"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K175027 short</partinfo> | <partinfo>BBa_K175027 short</partinfo> | ||
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Restriction site for the homing endonuclease I-SceI. | Restriction site for the homing endonuclease I-SceI. | ||
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Sequence will be cut as follows: | Sequence will be cut as follows: | ||
| − | 5' A G T T A C G C T A G G G A T A A^C A G G G T A A T A T A G 3' | + | 5' A G T T A C G C T A G G G A T A A^C A G G G T A A T A T A G 3'<br> |
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3' T C A A T G C G A T C C C^T A T T G T C C C A T T A T A T C 5' | 3' T C A A T G C G A T C C C^T A T T G T C C C A T T A T A T C 5' | ||
Revision as of 08:36, 1 August 2009
I-SceI restriction site
Restriction site for the homing endonuclease I-SceI.
Sequence will be cut as follows:
5' A G T T A C G C T A G G G A T A A^C A G G G T A A T A T A G 3'
3' T C A A T G C G A T C C C^T A T T G T C C C A T T A T A T C 5'
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]