Difference between revisions of "Part:BBa K143010"

Latest revision as of 11:09, 30 July 2009

Promoter ctc for B. subtilis

Promoter ctc is a sigma factor B-dependent promoter found in B. subtilis. In B. subtilis endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol1. The promoter ctc has been used previously as a read out for the activation of sigma factor B 2. The context with which we used the promoter ctc, was to take blue light as an input and give Polymerase Per Second(PoPS) as an output. To do this the other potential inputs need to be carefully controlled so that only blue light activates the sigma B and gives a PoPS output. In order to get sufficient sigma B activation by blue light the light receptor YtvA (BBa_K143037) needs to be significantly over expressed in B.subtilis 3.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

1 pmid=16267279
2 pmid=3100810
3 pmid=17575448

</biblio>

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 Promoter ctc is a sigma factor B-dependent promoter found in ''B. subtilis''. In ''B. subtilis'' endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol<cite>1</cite>. The promoter ctc has been used previously as a read out for the activation of sigma factor B <cite>2</cite>.
-The context with which we used the promoter ctc, was to take blue light as an input and give '''Polymerase Per Second'''(PoPS) as an output. To do this the other potential inputs need to be carefully controlled so that only blue light activates the sigma B and gives a PoPS output. In order to get sufficient sigma B activation by blue light the light receptor YtvA (<bbpart>BBa_K143037</bbpart>) needs to be over expressed in ''B.subtilis'' <cite>3</cite>.
+The context with which we used the promoter ctc, was to take blue light as an input and give '''Polymerase Per Second'''(PoPS) as an output. To do this the other potential inputs need to be carefully controlled so that only blue light activates the sigma B and gives a PoPS output. In order to get sufficient sigma B activation by blue light the light receptor YtvA (<bbpart>BBa_K143037</bbpart>) needs to be '''significantly''' over expressed in ''B.subtilis'' <cite>3</cite>.
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