Difference between revisions of "Part:BBa K4137000"
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<partinfo>BBa_K4137000 short</partinfo> | <partinfo>BBa_K4137000 short</partinfo> | ||
| − | a | + | BBa_K4137000, nicknamed ccdB for the purpose of our project, encodes for the production of CcdB protein which is a toxin to many bacteria. CcdB loses its toxicity by binding with CcdA, which prevents it from binding and interact with DNA Gyrase. The Myc tag was added to the CcdB coding sequence during the part design, this allows us to characterize the protein and go through purification. |
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Revision as of 07:30, 9 September 2022
CcdA-6xHis Antitoxin
BBa_K4137000, nicknamed ccdB for the purpose of our project, encodes for the production of CcdB protein which is a toxin to many bacteria. CcdB loses its toxicity by binding with CcdA, which prevents it from binding and interact with DNA Gyrase. The Myc tag was added to the CcdB coding sequence during the part design, this allows us to characterize the protein and go through purification.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]