Difference between revisions of "Part:BBa K4477005:Design"
(→Design Notes) |
|||
| Line 9: | Line 9: | ||
Amino acid sequence reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle). | Amino acid sequence reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle). | ||
| − | + | His tag and TEV cleavage sites were included upstream of the antibody. The his tag ensured the entire polypeptide could be purified out of a total protein solution using immobilized metal ion affinity chromatography (IMAC), and the TEV cleavage site ensure that TEV could be used to cleave off the his tags once they were no longer needed. | |
===Source=== | ===Source=== | ||
Revision as of 01:52, 7 September 2022
Camelid anti-mouse VHH antibody fragment
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Amino acid sequence reverse transcribed and codon optimized for expression in E. coli B (the parent strain of SHuffle).
His tag and TEV cleavage sites were included upstream of the antibody. The his tag ensured the entire polypeptide could be purified out of a total protein solution using immobilized metal ion affinity chromatography (IMAC), and the TEV cleavage site ensure that TEV could be used to cleave off the his tags once they were no longer needed.
Source
https://rupress.org/jcb/article/217/3/1143/39133/A-toolbox-of-anti-mouse-and-anti-rabbit-IgG