Difference between revisions of "Part:BBa K4390002"

 
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<partinfo>BBa_K4390002 short</partinfo>
 
<partinfo>BBa_K4390002 short</partinfo>
  
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This construct assembled using JUMP is a working transcriptional unit for the transcriptional repressor ArsR. This compound part is used to transform cells to express ArsR, so that the cell lysate contains ArsR and can be used in our biosensor.
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BBa_K4390002 is a working transcriptional unit which expresses the ArsR repressor (BBa_J15101). It is composed of the constitutive promoter J23100  (BBa_J23100), a ribosome binding site (BBa_B0034), the arsR repressor sequence (BBa_J15101) and the weak synthetic L2U2H09 Terminator (BBa_K4390001). This unit was assembled using JUMP and can be assembled into a pJUMP29-1A KanR Type IIS Level 1 vector plasmid for expression in cells.  
  
 
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Revision as of 16:25, 6 September 2022


ArsR expression construct

BBa_K4390002 is a working transcriptional unit which expresses the ArsR repressor (BBa_J15101). It is composed of the constitutive promoter J23100 (BBa_J23100), a ribosome binding site (BBa_B0034), the arsR repressor sequence (BBa_J15101) and the weak synthetic L2U2H09 Terminator (BBa_K4390001). This unit was assembled using JUMP and can be assembled into a pJUMP29-1A KanR Type IIS Level 1 vector plasmid for expression in cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 196
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]