Difference between revisions of "Part:BBa K4390001"
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|+ Loop Free-Energy Decomposition
 
|+ Loop Free-Energy Decomposition
 
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! Structural element !! δG !! Information
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! Structural element !! ΔG !! Information
 
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| External loop || -0.86 || 15 ss bases & 1 closing helices
 
| External loop || -0.86 || 15 ss bases & 1 closing helices

Revision as of 14:45, 5 September 2022


L2U2H09 Terminator

L2U2H09 is a synthetic terminator. The origin is https://www.addgene.org/127042/


L2U2H09 Structure.png

L2U2H09 structural information

This structure of L2U2H09 was generated using mFold DNA folding Form on the UNAFold Web Server. This structure was the likeliest based on loop free-energy predictions, with ΔG = -11.51.

Loop Free-Energy Decomposition
Structural element ΔG Information
External loop -0.86 15 ss bases & 1 closing helices
Stack -1.00 External closing pair is a4-t25
Stack -1.44 External closing pair is a5-t24
Stack -2.17 External closing pair is c6-g23
Stack -1.84 External closing pair is g7-c22
Stack -2.24 External closing pair is g8-c21
Stack -1.84 External closing pair is c9-g20
Stack -1.84 External closing pair is c10-g19
Stack -1.28 External closing pair is c11-g18
Helix -13.65 9 base pairs
Hairpin loop 3.00 Closing pair is t12-a17

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]