Difference between revisions of "Part:BBa K4390001"
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==L2U2H09 structural information== | ==L2U2H09 structural information== | ||
| + | {| class="wikitable" style="margin:auto" | ||
| + | |+ Loop Free-Energy Decomposition | ||
| + | |- | ||
| + | ! Structural element !! δG !! Information | ||
| + | |- | ||
| + | | External loop || -0.86 || 15 ss bases & 1 closing helices | ||
| + | |- | ||
| + | | Stack || -1.00 || External closing pair is a<sub>4</sub>-t<sub>25</sub> | ||
| + | |- | ||
| + | | Stack || -1.44 || External closing pair is a<sub>5</sub>-t<sub>24</sub> | ||
| + | |- | ||
| + | | Stack || -2.17 || External closing pair is c<sub>6</sub>-g<sub>23</sub> | ||
| + | |- | ||
| + | | Stack || -1.84 || External closing pair is g<sub>7</sub>-c<sub>22</sub> | ||
| + | |- | ||
| + | | Stack || -2.24 || External closing pair is g<sub>8</sub>-c<sub>21</sub> | ||
| + | |- | ||
| + | | Stack || -1.84 || External closing pair is c<sub>9</sub>-g<sub>20</sub> | ||
| + | |- | ||
| + | | Stack || -1.84 || External closing pair is c<sub>10</sub>-g<sub>19</sub> | ||
| + | |- | ||
| + | | Stack || -1.28 || External closing pair is c<sub>11</sub>-g<sub>18</sub> | ||
| + | |- | ||
| + | ! Helix !! -13.65 !! 9 base pairs | ||
| + | |- | ||
| + | | Hairpin loop || 3.00 || Closing pair is t<sub>12</sub>-a<sub>17</sub> | ||
| + | |} | ||
|} | |} | ||
Revision as of 14:41, 5 September 2022
L2U2H09 Terminator
L2U2H09 is a synthetic terminator. The origin is https://www.addgene.org/127042/
 |
L2U2H09 structural information
|
| Structural element | δG | Information |
|---|---|---|
| External loop | -0.86 | 15 ss bases & 1 closing helices |
| Stack | -1.00 | External closing pair is a4-t25 |
| Stack | -1.44 | External closing pair is a5-t24 |
| Stack | -2.17 | External closing pair is c6-g23 |
| Stack | -1.84 | External closing pair is g7-c22 |
| Stack | -2.24 | External closing pair is g8-c21 |
| Stack | -1.84 | External closing pair is c9-g20 |
| Stack | -1.84 | External closing pair is c10-g19 |
| Stack | -1.28 | External closing pair is c11-g18 |
| Helix | -13.65 | 9 base pairs |
| Hairpin loop | 3.00 | Closing pair is t12-a17 |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]