Difference between revisions of "Part:BBa K175003"
Tim Weenink (Talk | contribs) |
Tim Weenink (Talk | contribs) |
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The cut will leave a 4bp overhang of ATAA. | The cut will leave a 4bp overhang of ATAA. | ||
| − | 5' A G T T A C G C T A G G G A T A A^C A G G G T A A T A T A G 3' | + | 5' A G T T A C G C T A G G G A T A A^C A G G G T A A T A T A G 3'<br> |
3' T C A A T G C G A T C C C^T A T T G T C C C A T T A T A T C 5' | 3' T C A A T G C G A T C C C^T A T T G T C C C A T T A T A T C 5' | ||
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Revision as of 18:29, 29 July 2009
R751 plasmid with trbK knockout
Restriction site for the homing endonuclease I-SceI.
The cut will leave a 4bp overhang of ATAA.
5' A G T T A C G C T A G G G A T A A^C A G G G T A A T A T A G 3'
3' T C A A T G C G A T C C C^T A T T G T C C C A T T A T A T C 5'
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]