Difference between revisions of "Part:BBa K4271000"

Revision as of 02:57, 28 August 2022

OPH (organophosphate hydrolase) + his-tag

Our experiment aims to resolve environmental eutrophication, which is caused by the abundance of organic phosphate and nitrogen in the water. OPH, a gene that encodes organophosphate hydrolase, paraoxon, a type of organic phosphate and insecticide. We use paraoxon as the substrate of OPH's degradation, after which it would be converted into diethyl phosphate (DEP) and p-nitrophenol (pNP). We use pNP as an indicator for spectrophotometric identification. his-tag is attached to the end of the OPH gene for the purification of the protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 1082
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1091
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 193
Illegal AgeI site found at 13
Illegal AgeI site found at 298
Illegal AgeI site found at 433
Illegal AgeI site found at 496
1000
COMPATIBLE WITH RFC[1000]

Revision as of 06:30, 27 August 2022 (view source) Registry (Talk \| contribs)		Revision as of 02:57, 28 August 2022 (view source) JulieLin (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K4271000 short</partinfo>		<partinfo>BBa_K4271000 short</partinfo>

−	Our experiment aims to resolve environmental eutrophication, which is caused by the abundance of organic phosphate and nitrogen in the water. OPH, a gene that encodes organophosphate hydrolase, paraoxon, a type of organic phosphate and insecticide. We use paraoxon as the substrate of OPH's degradation, after which it would be converted into diethyl phosphate (DEP) and p-nitrophenol (pNP). We use pNP as an indicator for spectrophotometric identification.	+	Our experiment aims to resolve environmental eutrophication, which is caused by the abundance of organic phosphate and nitrogen in the water. OPH, a gene that encodes organophosphate hydrolase, paraoxon, a type of organic phosphate and insecticide. We use paraoxon as the substrate of OPH's degradation, after which it would be converted into diethyl phosphate (DEP) and p-nitrophenol (pNP). We use pNP as an indicator for spectrophotometric identification. his-tag is attached to the end of the OPH gene for the purification of the protein.