Difference between revisions of "Part:BBa K4348000"

 
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<partinfo>BBa_K4348000 short</partinfo>
 
<partinfo>BBa_K4348000 short</partinfo>
  
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An enzyme that converts cholesterol to 4-cholesten-3-one. It was discovered in Eubacterium coprostanoligenes by a team at the Broad Institute of MIT in 2021. This conversion is the first of the three-step pathway of converting cholesterol to coprostanol, a sterol that does not get absorbed by the gut. A 6x his-tag is attached to the N-terminal end, allowing for easy purification and analysis through nickel/cobalt columns and western blotting.
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A human 5-beta reductase used in bile acid synthesis. Used in the 2022 McGill project to catalyze the second step of the cholesterol -> coprostanol pathway, which is 4-cholesten-3-one to coprostanone. Coprostanol cannot be absorbed by the gut, which is a unique property.
  
 
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Revision as of 17:48, 22 August 2022


AKR1D1_his

A human 5-beta reductase used in bile acid synthesis. Used in the 2022 McGill project to catalyze the second step of the cholesterol -> coprostanol pathway, which is 4-cholesten-3-one to coprostanone. Coprostanol cannot be absorbed by the gut, which is a unique property.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]