Difference between revisions of "Part:BBa R0010"
| Line 11: | Line 11: | ||
* LacI can be inhibited by [http://openwetware.org/wiki/IPTG IPTG]. | * LacI can be inhibited by [http://openwetware.org/wiki/IPTG IPTG]. | ||
* LacI is coded by <bbpart>BBa_C0010</bbpart> | * LacI is coded by <bbpart>BBa_C0010</bbpart> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
Revision as of 19:52, 23 July 2009
promoter (lacI regulated)
This part is an inverting regulator sensitive to LacI and CAP.
It contains two protein binding sites. The first binds the CAP protein, which is generally present in E.coli and is asocciated with cell health and availability of glucose. The second binds LacI protein.
- In the absence of LacI protein and CAP protein, this part promotes transcription.
- In the presence of LacI protein and CAP protein, this part inhibits transcription.
- LacI can be inhibited by [http://openwetware.org/wiki/IPTG IPTG].
- LacI is coded by BBa_C0010
Usage and Biology
This is a direct copy of bases 0365739 through 0365540 of the E. coli K-12 MG1655 genome, Genbank NC_000913 in reverse complement form. It is the natural promoter for the LacZYA operon. It includes the tail end of the LacI gene coding region, but no promoter region for that partial gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]